Open AccessTransformation of immature wheat germ (Triticum aestivum L.) by particle bombardment
Author(s) -
A A Verbitskaya,
Anna Ivanova,
O. А. Shulga,
O A Schuklina,
A. K. Gaponenko
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/843/1/012042
Subject(s) - transformation (genetics) , photoperiodism , biology , wheat germ , autoclave , horticulture , botany , agronomy , chemistry , food science , gene , biochemistry , organic chemistry
The aim of the research was to create an effective method for producing transgenic wheat plants suitable for a wide range of promising varieties, both spring and winter crops. The plant material was cultivated at temperatures ranging from 4 to 25°C, either in the dark or in the light, with a 16-hour photoperiod (16/8 - day / night). Osram L36/77 FLUORA and F36W/33 Cool White lamps were used for lighting. The composition of all nutrient media included macroand micro-salts, vitamins B5, phytohormones and carbohydrates. The pH of the medium was adjusted to 5.8 before autoclaving. The medium was sterilized in an autoclave at pressure of 1.2 atmospheres for 15 minutes. An effective method of regeneration of transgenic wheat plants for ballistic transformation has been developed. Plants obtained by this method are phenotypically normal and fully fertile. The transgenic insertion of the target gene is transmitted to the offspring in accordance with Mendel’s laws. The transformation efficiency was high for all the studied varieties and ranged from 1.4 to 7.8%.