Open AccessKanamycin application features for the selective screening of genetically modified Populus × berolinensis
Author(s) -
В. В. Павличенко,
М. В. Протопопова
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/839/4/042020
Subject(s) - kanamycin , transgene , genetically modified crops , selection (genetic algorithm) , biology , gene , nutrient , botany , microbiology and biotechnology , genetics , computer science , ecology , artificial intelligence
The study describes the features of the kanamycin application for the effective selection of transgenic poplar expressing nptII gene. It has been shown that kanamycin concentration of 50 mg L −1 in the nutrient medium is the most suitable concentration for the effective selection of transgenic Berlin poplar plants for both regeneration and rooting stages. At the same time, 50 mg L −1 of kanamycin in the nutrient medium may result in the loss of the less resistant transgenic plants, so lower concentrations may be also applied. Kanamycin concentrations of 25 and 35 mg L −1 can give a false positive signal of transgenesis. Transgenic poplars expressing nptII gene can be effectively regenerated on nutrient media with kanamycin at concentrations up to 100 mg L −1 .