Open AccessIsolation, Population, and Selection of Phosphate Solubilizing Bacteria from Acid Soils of Institut Teknologi Sumatera’s Region, Lampung
Author(s) -
Muhammad Asril,
Yuni Lisafitri,
Ainin Niswati,
Suskandini Ratih Dirmawati
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/830/1/012016
Subject(s) - phosphate , ultisol , phosphate solubilizing bacteria , bacteria , population , soil water , veterinary medicine , nutrient , soil ph , soil test , biology , horticulture , chemistry , food science , rhizobacteria , biochemistry , rhizosphere , ecology , medicine , demography , sociology , genetics
Ultisol, which is acidic soil, is widely distributed in Indonesia, especially Sumatera Island. The characteristics of acidic ultisol make the presence of phosphate available in the soil deficient. This condition is caused by soil pH, which is very influential in phosphate availability in the soil. In acidic conditions, phosphate (P) binds to Fe or Al. Plants highly need the availability of phosphate in the soil. This condition is because phosphate is a nutrient that is needed by plants. Phosphate solubilizing bacteria are soil microbes that can improve P’s availability in acid soils by dissolving P that is bound to other metals. This study aims to obtain indigenous phosphate solubilizing bacteria from acid soils located in the Institut Teknologi Sumatera (ITERA). The study was conducted from April to June 2019, which included isolation of phosphate solubilizing bacteria from the soil, a test of the ability of phosphate dissolution on Pikovskaya medium, and pathogenicity test on potato tubers. The results showed that there were 35-74 x 105 cfu/g of soil samples. Seventeen isolates were obtained, which were able to use phosphate in the medium. Of the seventeen isolates, there were four potential isolates with the highest phosphate solubility index, namely EF.NAP 1 (1.2143), EF.NA3 (1,100), EF.NAP 4 (0.8616) and EF.NAP 9 (0.7188). Isolates have the best phosphate solubilizing index on the 6th day to the 7th day. The four potential isolates were not pathogenic in pathogenicity testing using potato tubers. These four isolates can be used to isolate candidates to improve the availability of phosphorus in the soil needed by plants in the ITERA region.