Open AccessThe use of coconut water (cocos nucifera l.) as alternative media to substitute Sabouraud Dextrose Agar (SDA) for the growth of aspergillus flavus
Author(s) -
Widhorini,
Asep Ginanjar Arip,
Astrini Wulandari
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/819/1/012061
Subject(s) - aspergillus flavus , agar , potato dextrose agar , cocos nucifera , food science , aspergillus , biology , significant difference , agar plate , horticulture , botany , mathematics , bacteria , statistics , genetics
Media is a material composed by mixed nutrition to grow microorganisms, common media to grow fungi is Sabouraud Dextrose Agar (SDA). It contains 10 g of epton, 40 g of dextrose and 15 g of agar. This study used various concentration of coconut water (20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and 100%. The aim of this study is to determine the concentration of coconut water that can be used to replace Sabouraud Dextrose Agar (SDA) for the growth of Aspergillus flavus and to determine the difference of colony diameter of Aspergillus flavus and to determine the difference of colony diameter of Aspergillus flavus. Macroscopic observation of colony diameter (mm) has been done in 1 x 24 hours for 10 days using calipers with Lactophenol Cotton Blue (SDA) dyes for affirmation test. Data was analyzed statistically using One Way Anova continued by Post hoc test. It showed that the higher the concentration of coconut water, the bigger the diameter colony (sig. 0,00 < 0,05). Post hoc test showed that coconut water concentration of 20%, 30%, 40%, 50%, and 60% gave lower significant growth of the fungus than Sabouraud Dextrose Agar (SDA) media, while the concentration of 70%, 80%, and 90% gave non – significant value or similar to wards control media Sabouraud Dextrose Agar (SDA). The concentration it can be concluded that coconut water can be used as an alternative media to substitute SDA (Aspergillus flavus) for the growth of Aspergillus flavus.