Sterility of 20 F1 genotypes derived from hybridization of several chilli’s lines with M1 male sterile

Open pollinated (OP) chili varieties seeds had been irradiated previously by gamma rays (400-600 Gy) to obtain four M 1 sterile plants for this study. These sterile plants could be used as a breeding material for F 1 male sterile hybrid variety. The purpose was to evaluate sterility of 20 F 1 genotypes derived from hybridization of several chili’s lines with four M 1 sterile plants. The research was conducted at Indonesian Vegetables Research Institute, Lembang (1,250 m above sea level) from April to December 2018. The research used a randomized complete block design (RCBD) with three replications. Population of each evaluated F 1 genotype was 10 plants/replication. Sterility trait evaluation was observed by fruit setting ability of various flower’s grouping positions (lower, middle, upper) on each F 1 genotype. Results show that there was one genotype categorized as sterile, one genotype as partial sterile, thirtheen genotypes as partial fertile, and five genotypes as fertile lines. Genotype 04 could be selected as a maintainer, whereas genotype RG-1 as a restorer line. Sterility of partial sterile lines with unstable trait could be increased by isolating and collecting the gene pool using back cross technique between their F 1 progenies with maintainer lines for at least five generations.