Open AccessEvaluation of crude ethanolic extract of aquatic plants as inhibition zone against Flavobacterium columnare
Author(s) -
Hessy Novita,
Media Fitri Isma Nugraha,
Rao Kn,
Mohamad Fadjar,
M. A. Radjamuddin
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/800/1/012018
Subject(s) - minimum inhibitory concentration , minimum bactericidal concentration , agar diffusion test , agar , flavobacterium , chemistry , ethanol , biology , microbiology and biotechnology , bacteria , antibacterial activity , antimicrobial , biochemistry , pseudomonas , genetics
Three different aquatic plants Selaginellaplana (Desv. Ex. Poir) Hieron, Ludwigiaadscendens (L.) H. Hara, dan Ephitemabenthamii C. B. Clarke were collected from South Sulawesi and crude extracts all of plants parts were prepared in ethanol. This extracts were checked for the presence of inhibitory zone by agar well diffusion method against gram negative Flavobacteriumcolumnare bacterial. Furthermore Minimum Inhibitory Concentration (MIC), Minimum bactericidal concentrations (MBC) testing has been done for crude extract of plants. Extracts of three plants prepared in ethanol showed relatively inhibitory zone to Flavobacterium columnareand two extracts of above plants were positive in MIC and MBC.