Open AccessAnalysis of Genome Characteristics of Helianthus annuus J-01 Chloroplast
Author(s) -
Jun Ma
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/792/1/012046
Subject(s) - genome , gene , biology , genetics , gc content , helianthus annuus , chloroplast , intron , transfer rna , codon usage bias , genome size , sunflower , rna , agronomy
Helianthus annuus J-01 was sequenced using a high-throughput sequencing platform, and the structural characteristics of the whole chloroplast genome sequence were analyzed. The results showed that the H. annuus J-01 chloroplast genome, like most higher plants, has a typical circular double-stranded tetrad structure. The chloroplast genome size is 151142bp, GC content is 38.99%, and a total of 127 genes have been annotated, including 84 protein-coding genes, 35 tRNA genes and 8 rRNA genes. A total of 18 genes in the H. annuus J-01 chloroplast genome contain introns, of which ycf3 and clpP genes contain 2 introns. In protein-coding genes, leucine is the amino acid with the highest codon encoding rate, and the amino acid with the lowest encoding rate is cysteine. The A/T content of the third codon is 69.32%, and the third codon of the H. annuus J-01 chloroplast genome coding gene prefers to use A/T.