Open AccessThe effect of salvia officinalis aqueous extract on generation number and time of leishmania tropica
Author(s) -
Neama Ali Ahmed,
Ban Yusif
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/790/1/012070
Subject(s) - petroleum ether , aqueous solution , chemistry , ethanol , salvia officinalis , sage , leishmania tropica , traditional medicine , chromatography , botany , extraction (chemistry) , nuclear chemistry , officinalis , biology , biochemistry , organic chemistry , medicine , physics , nuclear physics , leishmaniasis , immunology , cutaneous leishmaniasis
The present study examined the toxic effect of aqueous extracts, ethanol and petroleum ether, Salvia Officinalis, on the promastigote of L. tropica parasites growing in Tobies medium with human blood added (15%). The results showed that the aqueous and alcoholic extract and petroleum ether at different concentrations used concentrations (0.05, 0.1, 0.15, 0.2) mg/cm3 clear toxic effect on these parasites through a gradual decrease in their number with an increase in the concentration of each extract for an inverse relationship during growth periods (96, 72, 48, and 24 hours. It also showed that the high concentration of extracts (0.2) mg/cm3 led to an activation of the anterior flagellar growth with rates (80.6, 84.5, 71.9%) of the aqueous extract, ethanol alcohol and petroleum ether respectively. Exposing the frontal flagellum of L. tropica to ICSO concentration of both aqueous, alcoholic and petroleum extract of sage and nettle plants during the 96-hour period led to a decrease in DNA by (49.5, 55.2, 13.6), respectively, and also to RNA by (74.7, 73.9, 23.3).