Open AccessGenotypic detection of GES and VEB extended spectrum β-lactamase among aerobic Gram-negative bacteria isolated from diabetic foot ulcers
Author(s) -
Najah Mahdi Lukey,
Fatima Moeen Abbas
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/790/1/012054
Subject(s) - morganella morganii , microbiology and biotechnology , acinetobacter baumannii , klebsiella oxytoca , biology , bacteria , gene , gram negative bacteria , acinetobacter , polymerase chain reaction , klebsiella pneumoniae , escherichia coli , antibiotics , pseudomonas aeruginosa , genetics
Objective : Gram-negative bacteria with Extended-Spectrum β-Lactamase (ESBL) genes are of concern due to their susceptibility to multi-drug resistance. The goal of this research is to investigate the gene coding of resistance of ESBLs encoded by the VEB gene and GES gene to modern β-lactams. Method : seventy wound swabs were taken from diabetic foot ulcer patients in period from October (2019) to February (2020). The collected samples were cultured on different media agar to identify by morphological, biochemical tests and Vitek 2 system. To determine VEB gene and GES gene using the methodology of Polymerase chain reaction (PCR) on the isolated bacteria. Result : a total of 50 Gram negative bacteria. The distribution of VEB gene was Proteus ssp. 7, Morganella morganii.ssp. 2, Klebsiella oxytoca 1 , Acinetobacter baumannii 2. While the GES gene distribution was Escherichia coli 1, Proteus ssp 1, Morganella morganii. ssp 4, Acinetobacter baumannii 1. Conclusion : The VEB gene and GES gene plays an important role in the resistance to new β-lactams of ESBL-producing isolates.