Open AccessDetection lipase gene of Pseudomonas aeruginosa from crude oil contaminated soil
Author(s) -
Tawoos Mohammed Kamel Ahmed
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/790/1/012051
Subject(s) - lipase , pseudomonas aeruginosa , microbiology and biotechnology , contamination , extracellular , food science , enzyme , carbon number , triacylglycerol lipase , bacteria , gene , crude oil , chemistry , biology , isolation (microbiology) , emulsion , biochemistry , organic chemistry , ecology , alkyl , genetics , petroleum engineering , engineering
The microbial lipases are industrially more substantial. The bacterial lipase enzymes can be extracellular and intracellular, and are extremely affected by bacterial nutrition and various physicochemical factors like temperature degree, pH, the sources of carbon and nitrogen, inorganic salts and agitation. The objective of current work is isolation and identify of P. aeruginosa from crude oil contaminated soil depending on PCR targeted lipA gene. 20g (15 samples) of crude oil contaminated soil were collected from north oil refineries/Kirkuk/Iraq. The isolates were identified according to microscopically diagnosis and colonial properties, biochemical tests, API-20E system with diagnosis by PCR technique based on gene lip A. PCR products by electrophoresis demonstrated only 8 isolates (53.33%) with positive results with lipA 558 from all isolates of P. aeruginosa .