Open AccessA single nucleotide polymorphism of CAPN1 gene region 3’UTR in Bali cattle
Author(s) -
Dairoh Dairoh,
Md. Jakaria,
Mokhamad Fakhrul Ulum,
Cece Sumantri
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/788/1/012017
Subject(s) - genetics , single nucleotide polymorphism , three prime untranslated region , biology , five prime untranslated region , polymorphism (computer science) , gene , untranslated region , genotype , messenger rna
Bali cattle ( Bos javanicus ), as Indonesian origin genetic resources, is domesticated from banteng (Bibos banteng). Bali cattle have the potential to be developed as producer of premium beef. Genes that have important role in meat quality are calcium-activated neutral protease genes, as known as calpains (CAPN). Calpains are classified as Ca 2+ dependent intracellular cysteine proteases, including the ubiquitously expressed μ-calpain (CAPN1) and m-calpain (CAPN2). The purpose of this study was to analyze single nucleotide polymorphism (SNP) region 3’UTR CAPN1 gene in beef cattle. Polymorphism of CAPN1 gene was analyzed by direct DNA sequencing method in 42 Bali cattle that compared with 11 Belgian Blue, 7 Limousine, 12 Pasundan, and 12 Katingan. The result showed that CAPN1 gene has 7 polymorphic SNPs (g.15284 C>T, g.15347 T>G, g.15525 G>A, g.15674 C>T, g. 15853 G>A, g.15905 G>A and g.15915 G>A) in Bali cattle. These SNPs that polymorphic in Bali cattle were monomorphics in Belgian Blue, Limousine, Pasundan and Katingan cattle. Only one SNP g.15853 G>A in Bali cattle was polymophics in Belgian Blue. Deletion was detected that 8 nucleotides deletion (CTCCCTCC) occurred in Bali, Pasundan, and Katingan cattle at position g.15795 – g.15802, while Belgian Blue and Limousine cattle the deletion was not found.