Anti-fatigue effect of enzymatic protein hydrolysate from Macadamia

The anti-fatigue effect of macadamia (Macadamia integrifolia) protein and hydrolysate prepared with basic protease were investigated. Random mice grouping was carried out to create 4 groups including the control group and protein hydrolysate groups at low (150 mg/kg·d), medium (500 mg/kg·d) and high (1500 mg/kg·d) doses. All groups were treated by gavage for 28 consecutive days. Weight-loaded swimming test, serum urea nitrogen, serum creatine kinase activity, blood lactic acid and liver glycogen assays were performed to assess its anti-fatigue effect. The results showed that macadamia protein hydrolysate could significantly prolong weight-loaded swimming time, promote synthesis of liver glycogen and decrease blood urea nitrogen and lactic acid. Moreover, it can also significantly reduce serum creatine kinase activity. Therefore, macadamia protein hydrolysate had significant anti-fatigue function.