Open AccessGrowth of thermotolerant Pichia kudriavzevii UniMAP 3-1 strain for ethanol production using xylose and glucose at different fermentation temperatures
Author(s) -
Khadijah Hanim Abdul Rahman,
Ku Syahidah Ku Ismail,
Nazalan Najimudin
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/765/1/012107
Subject(s) - xylose , fermentation , yeast , food science , ethanol fuel , xylitol , ethanol , chemistry , hydrolysis , pichia stipitis , biochemistry
The applications of thermotolerant microorganisms in the production of lignocellulosic bioethanol is the key factor for successful simultaneous saccharification and fermentation process. Thus, this study aimed to isolate a thermotolerant yeast strain that was able to convert both glucose and xylose into ethanol. An analysis based on D1/D2 region of the large-subunit ribosomal DNA identified the isolated strain namely as Pichia kudriavzevii UniMAP 3-1. The growth of this newly isolated yeast was tested with fermentation temperature at 30°C and 40°C on xylose and glucose. P. kudriavzevii UniMAP 3-1 was able to ferment xylose to ethanol at both 30°C and 40°C with a yield of 0.013 g/g and 0.019 g/g with concomitant xylitol yield of 0.24 g/g and 0.25 g/g, respectively. Fermentation of glucose to ethanol was also tested at 30°C and 40°C and the yields were 0.42 g/g and 0.41 g/g, respectively. The potential of this thermotolerant yeast to be used in high-temperature fermentation in both glucose and xylose are proven in this study.