Open AccessHydrogel synthesis of PVA/O. sativa for antimicrobial activity using freeze thaw method
Author(s) -
Che Wan Sharifah Robiah Mohamad,
Amri Abdulah,
Khairul Farihan Kasim,
M. A. Sukhri
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/765/1/012038
Subject(s) - antimicrobial , agar diffusion test , agar , chemistry , phytochemical , antibacterial activity , compatibility (geochemistry) , bacteria , food science , materials science , biochemistry , organic chemistry , biology , composite material , genetics
This research project was aimed to develop antibacterial hydrogel from blends of PVA and Oryza sativa L by applying freeze-thaw method (later on will refer as PVA/OS hydrogel). Agar well diffusion method was used to test the antimicrobial activity of the hydrogel against Gram-negative ( E. coli ) and Gram-positive bacteria ( Staphy. aureus ). The results indicate that PVA 20 /OS at pH 7 was effective as antimicrobial against both tested organisms while OS/PVA20 at pH 8 was effective for E. coli only. Based on the phytochemical test, the antimicrobial activity probably causes by the present of flavonoids content in the hydrogel. The higher PVA used in the blend’s hydrogel will increase the thickness of hydrogel. PVA40 at pH 7 and pH8 shows a good result in mechanical properties where the value of thickness is 0.750 and 0.770 mm respectively. PVA/OS blend hydrogel had good antimicrobial activity and good compatibility to form an antimicrobial hydrogel for medical purpose with the used of 20%.