Open AccessAntioxidant activity of methanolic extract of Eucheuma spinosum extracted using a microwave
Author(s) -
Ni Luh Putu Mita Rismayanti,
Amir Husni
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/763/1/012028
Subject(s) - phytochemical , flavonoid , dpph , stratum spinosum , chemistry , antioxidant , extraction (chemistry) , food science , traditional medicine , botany , chromatography , biology , biochemistry , medicine , stratum corneum , genetics
Eucheuma spinosum is a widely cultivated red seaweed. The phenolic compounds contained in E. spinosum , especially flavonoids, can be used as a source of natural antioxidants. This study aimed to determine the antioxidant activity of E. spinosum extracted using a microwave with different extraction times (6, 8 and 10 minutes). The parameters measured included yield, phytochemical test, total phenolic content, total flavonoid content and antioxidant activityt, namely 2,2 diphenyl-1-picrihydrazyl (DPPH) and ferric reducing antioxidant power (FRAP). The results showed that the E. spinosum extract with an extraction time of 10 minutes had the highest yield with a total phenolic content of 59.42 ± 27.36 mg GAE/g and a total flavonoid content of 65.48 ± 25.34 mg QE/g. The results of phytochemical screening showed that the extract contain triterpenoid compounds. The antioxidant activity of the E. spinosum extract showed the highest IC 50 value of 46.52 ± 5.53 ppm using the DPPH method and the FRAP method was 58.92 ± 1.76 μM/g.