Open AccessUsing in vitro technique for drought, heat, and combined drought-heat assay in potatoes
Author(s) -
Titik Handayani,
Kazuo Watanabe
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/762/1/012061
Subject(s) - wilting , drought stress , abiotic component , abiotic stress , heat stress , horticulture , drought tolerance , agronomy , biology , zoology , ecology , biochemistry , gene
Drought and heat stresses are the significant abiotic stresses threatening the food crops, including potato. The in vitro technique is promising to conduct an abiotic stress assay. The assay of drought, heat, and combined drought-heat stress was conducted by using five potato lines. Polyethylene glycol (PEG) 8000 (25%) was used to induce drought stress. Heat stress was obtained by growing the plantlets at 35°C. Whereas placing the plantlets cultured in the media containing PEG 8000 (25%) at 35°C was applied to generate combined drought-heat stress. The results showed that four hours of abiotic treatments had no effect on growth of potato plantlets. The results showed that potato lines gave different responses to drought and combined drought-heat stress. Potato line 87HW13.7 wilted 8 hours after drought and combined drought-heat treatments, which became more severe by the time and died after 48 hours on combined drought-heat stress. Potato line 84.194.30 showed the wilting symptom after 24 hours due to drought stress and combined drought-heat stress and performed less wilting among five potato lines. The in vitro technique could differentiate the response of different potato lines to abiotic stress; therefore, this method would help the abiotic stress assay in potatoes.