Open AccessThe effect of medium formulation and BAP concentration of growth and development of Aquilaria malaccensis Lam. shoot in vitro
Author(s) -
Betalini Widhi Hapsari,
. Rudiyanto,
Andri Fadillah Martin,
Tri Muji Ermayanti
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/762/1/012033
Subject(s) - shoot , agarwood , explant culture , horticulture , murashige and skoog medium , botany , biology , micropropagation , chemistry , in vitro , medicine , biochemistry , alternative medicine , pathology
Tissue culture of agarwood tree ( Aquilaria malaccensis Lam) was done to produce a large number of transplants in the laboratory. The compositions of medium culture are commonly modified to obtain the best growth of explants to regenerate into plantlets. Our study aimed to evaluate and compare different medium formulations supplemented with BAP on agarwood shoot culture growth. In this study we used MS, NN, B5, AR and WP medium containing 0 (control treatment), 0.25, 0.5, 1.0 and 2.0 mg/L BAP. The shoot tips were cultured for 16 weeks in the treatment media. Height of shoots, number of leaves, number of adventive shoots, and number of roots was recorded every week to determine the growth. The results showed that after 16 weeks of culture, the best medium for shoot height was NN medium with BAP fortification (3.54cm) for number of leaves and adventive shoots proliferation was Gamborg B5 medium fortified with 0.5 mg/L BAP (21.63 leaves and 8,38 shoots), while NN medium without BAP was best for rooting.