Open AccessIsolation and Identification of Alkaline Protease Producing Aspergills niger from Iraqi Soils
Author(s) -
Z. T. Sedrah,
Elham I. Al-Shamary,
S. Kh. Nassri
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/761/1/012117
Subject(s) - aspergillus niger , alkaline protease , protease , biology , aspergillus , gene , microbiology and biotechnology , isolation (microbiology) , 16s ribosomal rna , strain (injury) , enzyme , biochemistry , anatomy
Twenty purified isolates were obtained by using different soil sources, only twelve isolates belonging to Aspergillus genera depending on cultural and morphological characterization. The isolates were used as alkaline protease producer. The highest proteolytic, enzymatic activity (95.83U/ml) was obtained from Aspergillus sp. ZE isolate. This isolate was identified by 5.8 rRNA gene sequencing as Aspergills niger (accuracy of 99%), which was matched with the sequence of Aspergills niger strain GM775228 recorded in Gene bank under the ID: GM 775228.1.