Open AccessA novel and Rapid Method for Detecting Bio-fungicide Properties in Plant Species
Author(s) -
R. M. Theer,
Ayad Alheeti,
Ayoob Obaid Alfalahi
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/761/1/012024
Subject(s) - macrophomina phaseolina , fungicide , biology , bioassay , charcoal , potato dextrose agar , agar , horticulture , botany , inoculation , chemistry , bacteria , organic chemistry , genetics
Searching for eco- friendly bio-fungicides have become promising strategy to confront the resistance phenomenon against synthetic fungicides in pathogenic fungi. Unlike the previously described techniques to survey the plants for their secondary toxic metabolites against fungi, a novel technique reported here. The new technique designing on using a detached leaf bioassay and inoculum of Macrophomina phaseolina , the charcoal rot pathogen of sesame. Of an eleven different plant species belong to different families examined with this technique only one species (Giradol, Chrozophora tinctoria of Euphorbiaceae) showed no response to Macrophomina phaseolina inoculation on detached leaf even with wounding. The aseptic aqueously extracted leaf of the Giradol inhibited the growth of the Macrophomina phaseolina by 90% on potato dextrose agar media. This technique is relatively easy, fast and enabling to evaluate for bio- fungicidal existence in several species of plant at the same time.