Open AccessMolecular and phenotypic evaluation for heading date and panicle length on F2 selected aromatic rice progenies derived from gene pyramiding effort
Author(s) -
o Carsono,
Yerisca Novri Nathania Saragih,
Santika Sari,
Meddy Rachmadi
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/759/1/012070
Subject(s) - panicle , biology , genotype , trait , aromatic rice , genetics , amylose , horticulture , microbiology and biotechnology , quantitative trait locus , molecular marker , gene , agronomy , oryza sativa , food science , starch , computer science , programming language
Gene pyramiding, an attempt to combine many favourable genes from many parents into one single genotype, has been applied in recent years. Through hybridization of many rice genotypes including cv. Pandanwangi (fragrance rice), PTB33 (brown planthopper resistant), Ciapus (high yield and moderate amylose content) and KA (early maturity), some promising genotypes have been obtained. Thus, in order to select the best genotypes among the F 2 progenies derived from gene pyramiding effort, both phenotypic and molecular evaluations have to be done. These pyramided genotypes were evaluated with IFAP and ESP ( fgr gene), RM19414 ( Hd3 ), RM7601 ( Hd2 ), and RM3600 ( LP1) for molecular assessment, meanwhile morpho-agronomic traits were done to assess their performance. Aromatic trait was detected by 1.7% KOH. Meanwhile heading date and panicle length were measured phenotypically. Data obtained from molecular markers were scanned by visualizing DNA bands and calculating PIC (Polymorphism Information Content). Data from phenotypic markers were categorized based on IBPGR (International Board for Plant Genetic Resources) standards. Genotype #131 was supposed to have high yield potential, early maturity, aromatic, and intermediate amylose content. The selected will be recommended as promising genotype and continued as elite breeding materials and will be evaluated for their benefit.