Open AccessInvestigation of a Proper Sampling Position for Freshness Assessment of Cabbage by Spectrophotometric-Chemo Metric Approach
Author(s) -
Desy Syukri,
Wellyalina,
Risma Nanda
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/757/1/012062
Subject(s) - layer (electronics) , extraction (chemistry) , chromatography , metric (unit) , chemistry , statistical analysis , sampling (signal processing) , biological system , mathematics , analytical chemistry (journal) , environmental science , statistics , computer science , biology , engineering , organic chemistry , operations management , filter (signal processing) , computer vision
The objective of this study was to identify the differences of cabbage’s layers based on their responses to the environmental conditions. The differences between layers were observed according to the spectrophotometric evaluation. The three outer layers of a fresh cabbage were separated, sampled and put into extraction tubes. The metabolites inside the each layer were extracted by the mixture of isopropanol and hexane (2:1) and analyzed by a spectrophotometer with a scanning analytical mode from 200 nm to 800 nm. The obtained data were analyzed with a metabolomics statistical software. Based on the statistical results, it can be concluded that each layer of cabbage has a specific profile of metabolites distribution. Each layer has produced different responses to the environment after harvest where the first layer indicated more sensitive to the environmental conditions. Therefore, the selection of the first layer of cabbage is not proper for any evaluation relating to postharvest investigations.