Open AccessScreening of antagoinistic fluorescent Pseudomonads from rice rhizosphere for growth suppression of Magnaporthe oryzae and their molecular identification
Author(s) -
M Z I Zihad,
Abeda Sultana,
Farzana Haque Tumpa,
Shila Chakraborty,
Md. Atiqur Rahman Khokon
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/756/1/012042
Subject(s) - pseudomonas fluorescens , biology , rhizosphere , pseudomonas , 16s ribosomal rna , xanthomonas oryzae , bacteria , microbiology and biotechnology , oryza sativa , pathogen , botany , gene , biochemistry , genetics
Isolation and identification of native fluorescent Pseudomonads from the rice rhizosphere were evaluated for their growth suppressing ability against blast disease of rice causing pathogen (Magnaporthe oryzae). Twenty strains of fluorescent Pseudomonads were isolated and identified initially based on their cultural and in vitro growth suppressing ability against M. oryzae. Among them BdPf-4, BdPf-8, BdPf-9 and BdPf-10 exhibited complete in vitro growth suppression (100%) against M. oryzae following dual culture in growth medium. Molecular identification of the antagonistic Pseudomonas fluorescens was done using 16S rDNA primers. Gel-electrophoresis of PCR products of all the isolates confirmed the fluorescent Pseudomonads showing band at 1500 bp. Phylogenetic analysis of the sequenced data revealed that BdPf-8 (MN256392.1), BdPf-9 (MN256393.1), BdPf-10 (MN256394.1) have 100% homology with Pseudomonas putida (MT184822.1) from India, Bacterium sp. (MK823484.1) from China, Pseudomonas sp. (KY324900.1) from Brazil respectively. Seed priming by different strains of P. fluorescens significantly increased vigor index of rice seedlings. The highest vigor index (2274.8) and (2211.6) which was 184.42% and 176.53% higher over control treatment was found in BdPf-16 (MN256399.1) and BdPf-10 (MN256394.1) respectively. These results revealed the possibility of potential use of some of the strains of native P. fluorescens for management of blast disease of rice.