Open AccessAntibacterial Potential of Nicotiana tabacum L. var Virginia Pyrolysis Extract Against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, and Pseudomonas aeruginosa
Author(s) -
Shanni Fernanda,
B. A. Amru,
Hossein Rahmani,
Misri Gozan,
N. S. Irsyad,
Meiskha Bahar,
Oktaviani Sandra Puspita,
Fajriati Zulfa,
Andri Pramesyanti Pramono
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/755/1/012013
Subject(s) - enterococcus faecalis , staphylococcus aureus , microbiology and biotechnology , escherichia coli , pseudomonas aeruginosa , nicotiana tabacum , biology , bacteria , chemistry , biochemistry , genetics , gene
Tobacco plants are one of the main trade commodities in Indonesia. At present, the main production of tobacco is cigarettes. However, tobacco has active antibacterial compounds, such as phenols, alkaloids, and essential oils. Therefore, tobacco can be used in the health sector. This study was conducted to determine the effectiveness of the pyrolysis extract of Nicotiana tabacum L. var Virginia in inhibiting Staphylococcus aureus , Enterococcus faecalis , Escherichia coli , and Pseudomonas aeruginosa . This study uses a true experimental research design with tobacco extract samples obtained by pyrolysis at concentrations of 20%, 40%, 60%, 80%, and 100%. The antibacterial test carried out was the Kirby-Bauer diffusion method on Mueller Hinton Agar (MHA) media. One-Way ANOVA test results with p < 0.05 indicate the effectiveness of tobacco pyrolysis extract in inhibiting Staphylococcus aureus , Enterococcus faecalis , Escherichia coli , and Pseudomonas aeruginosa . The average yield of inhibition zones found in Staphylococcus aureus , Enterococcus faecalis , Escherichia coli , and Pseudomonas aeruginosa were 6.35 mm, 5.9 mm, 3.97 mm, and 5.025 mm. From these results, Staphylococcus aureus bacteria became the most sensitive bacteria with Virginia tobacco pyrolysis extract.