Open AccessThe Effect of Weight and Incubation Time in DNA Quality of Kepel (Stelechocarpus burahol) Leaves
Author(s) -
Etty Handayani,
Nandariyah,
Vita Ratri Cahyani,
- Parjanto
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/752/1/012017
Subject(s) - germplasm , dna , dna extraction , agarose gel electrophoresis , incubation , genetic diversity , biology , isolation (microbiology) , incubation period , horticulture , agarose , gel electrophoresis , polymerase chain reaction , botany , microbiology and biotechnology , biochemistry , gene , medicine , population , environmental health
Kepel ( Stelechocarpus burahol ) is a rare fruit plant which becomes the identity plant of the Yogyakarta Region. Kepel plants need to be conserved so they don’t become extinct Germplasm of kepel must be collected to get the genetic diversity of Kepel . Research on the genetic diversity of Kepel plants has never been done, so it needs the right method to obtain good quality DNA for molecular studies for future research. This study aims to obtain the best sample weight and incubation time to produce high-quality DNA for future research. This study used samples from young green leaves of kepel . DNA was extracted and purified from the leaves using a modified CTAB method to obtain DNA of kepel with high-purity and high-concentration. The result of DNA isolation and purification was analyzed by spectrophotometer and agarose gel electrophoresis method. The yield of DNA isolation on young kepel leaves had higher quality and quantity than DNA isolation on mature kepel leaves The results demonstrated that DNA isolation from young green leaves of kepel had a purity level of 1.64 – 2.01. In addition, it also showed that DNA isolation from young green leaves in weight 0.2 g with 30 minutes incubation gave the highest DNA concentration (880 ng/µl).