Open AccessScreening of Extracellular Enzyme from Phaeophyceae-Associated Fungi
Author(s) -
E P Ayuningtyas,
Mada Triandala Sibero,
Nadia Elisabet Br. Hutapea,
E H Frederick,
Retno Murwani,
Dewi Seswita Zilda,
Diah Permata Wijayanti,
Agus Sabdono,
Delianis Pringgenies,
Ocky Karna Radjasa
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/750/1/012005
Subject(s) - enzyme , amylase , extracellular , enzyme assay , starch , fermentation , microfungi , chemistry , biochemistry , biology , agar plate , microbiology and biotechnology , food science , botany , bacteria , genetics
Enzymes are known as a multipurpose catalyst and their potential through time. Various types of enzymes are widely utilized in fermentation and the pharmaceutical industry. One of the most potential enzyme producers is marine fungi, which can be isolated from seaweed. The purposes of this research were to isolate and screen seaweed-associated fungi that exhibited extracellular amylase. The fungi were isolated by tapping method on PDA medium and screened using starch agar, agarase, alginate, and carrageenan medium. Enzyme activity was detected by flooding the starch plates with iodine reagent. The presence of the inhibition zone around the colony indicated enzyme activity. Two potential isolates were identified as Penicillium oligosporum . and showed their activity in producing amylase, agarase, alginate lyase, and carrageenase.