Open AccessCaffeine-degrading endosymbiont fungi isolated from Hipothenemus hampei. Ferr as pre-analysis caffeine tolerance ability of coffee berry borer
Author(s) -
Purwatiningsih,
Sy. Ubaidillah,
Dwi Setyati,
Hidayat Teguh Wiyono
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/743/1/012088
Subject(s) - berry , caffeine , biology , botany , agar , horticulture , agar plate , pest analysis , food science , bacteria , genetics , endocrinology
The Coffee Berry Borer (CBB), Hypthenemus hampei Ferr., is the most destructive pest of coffee berry due to its ability to bore and complete their entire life cycle inside of the coffee berry. The detoxification mechanism of CBB ability to lives inside of coffee berry containing high natural insecticide compound such as caffeine is a very interesting study and it may lead to be an advantaged for CBB controlling methods. Isolation and screening of endosymbiont fungi from CBB using 0.5% caffeine plate agar as a minimum nutrient as well as a selective medium were obtained 10 isolates consist of 6 isolates of yeasts and 4 filamentous fungi which positively degraded caffeine content through their formed diameter of the colony. The presenting of these several caffeine-degrading endosymbiont fungi could be as pre-explanation about the caffeine tolerance mechanism of CBB.