Open AccessMolecular identification of Uncaria gambir [Hunter] Roxb. through DNA barcoding
Author(s) -
Epi Supri Wardi,
Sumaryati Syukur,
Zulkarnain Chaidir,
J. Jamsari,
Verawati,
S. K. Mukti
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/741/1/012056
Subject(s) - dna barcoding , biology , internal transcribed spacer , traditional medicine , botany , barcode , ribosomal rna , genetics , evolutionary biology , gene , medicine , computer science , operating system
Gambir [ Uncaria gambir [Hunter] Roxb.] is an important source of traditional medicinal from West Sumatera. Different types of this plant give different catechin content as one of the active compounds. The identification of the correct species and types are important for quality control of medicinal materials. The internal transcribed spacer [ITS] of nuclear ribosomal DNA is one of the most commonly used DNA markers in plant phylogenetic and DNA barcoding analyses, and it has been recommended as a core plant DNA barcode. Four types of gambir [Riau Gadang, Mancik, Cubadak, and Udang] and ITS barcode named ITS4 and ITS5 were used in this study. This barcode gives 100% amplification efficiency and length of products 592-690 bp. variation sites detected for gambir Cubadak and gambir Riau Gadang. The results are able to discriminate 2 types in Uncaria gambir [Hunter] Roxb. Species which are essential for the investigation of adulterants and misidentifications of gambir.