Open AccessSimulated mutations in the geminivirus replicase gene using in-silico CRISPR / Cas9-based methods
Author(s) -
Ridha Hidayati,
Bastian Nova,
D. Cania,
T. Runifah,
Djong Hon Tjong,
J Jamsari
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/741/1/012043
Subject(s) - crispr , biology , gene , in silico , rna dependent rna polymerase , cas9 , genome editing , genome , genetics , viral replication , virology , computational biology , virus , rna
Geminivirus is one of the causes of losses in the agricultural sector. This virus infects many horticultural crops such as tomatoes, potatoes, eggplant, cucumbers, and chilies. All genes in geminivirus have an important and interrelated role in the success of infection. Replicase is a gene that is key in the replication of the viral genome in its host. The occurrence of mutations in this gene is thought to stop the virus from multiplying in the host. This will lead to the failure of the virus to infect the plant. To achieve this, an in-silico mutation simulation can be performed first. Mutations were simulated following the CRISPR / Cas9 method using Phyre2, ProFunc, CASTp, and Chimera software. This simulation succeeded in predicting that the CRISPR / Cas9 system on the replicase gene could inhibit viral replication. The success of this simulation will become a reference and consideration for CRISPR/Cas9 further research at the laboratory scale.