Open AccessCloning of gyrB housekeeping gene from Serratia plymuthica UBCF_13
Author(s) -
Nurefni Azizah,
R Fatiah,
J Jamsari
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/741/1/012042
Subject(s) - housekeeping gene , amplicon , plasmid , gene , biology , cloning (programming) , genetics , puc19 , genome , computational biology , polymerase chain reaction , computer science , gene expression , programming language
Serratia plymuthica strain UBCF_13 is a biocontrol agent that has the potential to inhibit pathogenic fungi. S. plymuthica has housekeeping genes, one of which is the gyrB gene. The housekeeping gene was cloned to be used as a template for standard curve creation regarding the correlation of gene copy number and Ct value in absolute quantification data. This study was conducted to provide the plasmid housekeeping gene gyrB from S. plymuthica which will be used as a template for standard curve creation in absolute quantification. The method used in this study were DNA genome isolation, PCR, ligation, and transformation. The result showed that the housekeeping gene gyrB has been successfully cloning based on the size of the estimated amplicon, which is 114 bp.