Open AccessThe effect of genotype on in vitro morphogenesis of blue- and purple-grained bread wheat
Author(s) -
A A Shkurkina,
L. P. Khlebova,
Yu V Melnikova,
V P Vistovskaya,
S E Mityukhina
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/723/2/022055
Subject(s) - callus , morphogenesis , biology , explant culture , in vitro , botany , grain yield , murashige and skoog medium , adaptation (eye) , horticulture , biochemistry , gene , neuroscience
Bread wheat containing anthocyanins in grain is of great interest for in vitro cell selection. These compounds are antioxidants and help the plant cope with stress. For the first time, we have evaluated the regenerative potential of eight samples with purple and blue grain using mature and immature embryos as explants. The maternal plants grew in the field conditions in the south of Western Siberia (Russia). Colored wheat had an advantage over white-grained forms in the ability to in vitro morphogenesis and adaptation to ex vivo conditions. Samples Fioletovozernaya (k-55583) and Blue A (k-43091) showed an intense proliferation of callus (94-95%), a high morphogenic activity (74-100%) and a large yield of viable regenerants (4-6 from one callus). We consider these forms promising for the selection of cell and plant lines that are resistant to adverse environmental factors and are suitable for breeding programs.