Open AccessA New Visible Spectrophotometric Approach for Mutual Determination of Allopurinol drug in Pharmaceuticals after Cloud Point Extraction
Author(s) -
Sahar S. Hassan,
Ihsan Mahdi Shaheed,
V. Noor Mohammed,
Saadiyah A. Dhahir
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/722/1/012033
Subject(s) - allopurinol , detection limit , extraction (chemistry) , cloud point , chromatography , chemistry , correlation coefficient , mathematics , pathology , medicine , statistics
A new spectrophotometric methodology for allopurinol (ALLO) drug determination in pharmaceuticals was a proved. The method is based on formation of allopurinol-CoII complex in alkaline medium. A non-ionic surfactant Triton X-114 was used for the extraction of this complex at 586 nm. High extraction for complex was maintained under optimized conditions. The linearity was performed form (5.0-35.0) μg mL -1 with correlation coefficient (R 2 =0.9995). Limit of detection (LOD) and limit of quantification (LOQ) were 0.393 and 1.308μg mL -1 , respectively. This new method was applied successfully for allopurinol determination in pharmaceuticals and maintained acceptable accuracy and precision.