Open AccessThe Study of Antiphagocytic Activity of Streptococcus Isolated from Cows During Mastitis and Endometritis
Author(s) -
M.N. Loschinin,
A.V. Gorbatov,
Н. А. Соколова,
Yury Isaev
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/720/1/012058
Subject(s) - mastitis , microbiology and biotechnology , streptococcus uberis , endometritis , biology , streptococcus dysgalactiae , streptococcus equi , phagocytosis , bacteria , streptococcus agalactiae , streptococcus , immunology , pregnancy , genetics
Streptococcal mastitis causes enormous economic damage in dairy farming. Milk loses its nutritional value due to the appearance of pathogenic bacteria in it that causes food toxicosis. In this study we used 28 isolates of streptococci isolated from cows with mastitis and endometritis. Of these, 38.9% belonged to S. agalactae, 33.3% to S. dysgalactae, and S. equi subsp. zooepidemicus - 22.2%. A small number of isolates -5.6% belonged to the species S. equi subsp. equi and S. uberis. in The test with mouse peritoneal macrophages shows that the antiphagocytic activity was 75% for isolates of S. zooepidemicus, 57% for S. agalactiae, and 64% for S. dysgalactiae. At the same time, the phagocytic number and phagocytic index were higher in the phagocytosis of the most strains featured with trypsin compared to the initial ones indicating to the antiphagocytic role of the pathogenic structures presented in them at the stage of bacteria capture. The numbers of factors of streptococci pathogenicity including M-proteins make them able to resist opsonization and phagocytosis