Open AccessDNA Sequencing of Bovine Leukemia Proviruses with Determination of the Genetic Status of Isolated Isolates
Author(s) -
N.G. Kozyreva,
Л. А. Иванова,
Татьяна Ивановна Степанова
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/720/1/012053
Subject(s) - bovine leukemia virus , biology , phylogenetic tree , gene , genetics , genotype , virology , population , clade , virus , demography , sociology
This paper presents data on the study of bovine leukemia virus (BLV) polymorphism in the Ulyanovsk region. A phylogenetic analysis of 33 field BLV isolates isolated from seropositive animals was performed. The nucleotide sequence of env and pol gene regions was determined. The study revealed heterogeneity of the studied BLV population by the env gene, which corresponds to 2 genotypes of the virus: IV (97%), VII/VIIA (3%) with confirmation of the dominance of the IV genetic variant of the pathogen in Russia, and homogeneity by the pol gene, expressed in clustering of all analyzed isolates with those representatives of the RRIEVM database and international isolates assigned to clade 1 (100%). The revealed nature and degree of genetic differences/divergences maintain the general trend in the RRIEVM database and do not exceed the existing range.