Open AccessApplication of Serum Free Medium Cultured Vero Cells for the Production of Recombinant Oncolytic Herpes Simplex Virus 2
Author(s) -
Zongyao Fang,
Leilan Wang,
Zongxing Zou,
Siqi Zhang,
Zhang Yin,
Sheng Hu,
Binlei Liu
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/714/3/032001
Subject(s) - oncolytic virus , vero cell , herpes simplex virus , virology , recombinant dna , cell culture , virus , biology , microbiology and biotechnology , gene , biochemistry , genetics
The oncolytic virus, especially the recombinant oncolytic herpes simplex virus 2 (oHSV2), has attracted much attention because of its promising cancer treatment effect. To eliminate animal-derived substances from serum supplemented culture medium, which is an adverse factor for the safety of biological products, this study selected three typical serum free media (SFM) on the market, and successfully obtained three SFM cultured Vero cell lines which could be expanded stably. This work provided a reference for the application of SFM to the production of other biological products or vaccine.