Open AccessMass spectrometry analysis revealed the production of siderophore from Klebsiella oxytoca strain STA01 isolated from sago palm field in Tulehu, Maluku, Indonesia
Author(s) -
Andri Frediansyah,
Saefuddin Aziz,
WA Ramadaningrum,
and Al Sunarwidhi
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/712/1/012011
Subject(s) - klebsiella oxytoca , siderophore , 16s ribosomal rna , biology , genbank , ribosomal rna , strain (injury) , microbiology and biotechnology , klebsiella pneumoniae , bacteria , gene , escherichia coli , anatomy , genetics , biochemistry
Klebsiella oxytoca STA01 produced the serratiochelin A, an NRPS based siderophore. The strain isolated from the sago palm field in Tulehu, Ambon, Maluku, Republic of Indonesia. Its partial sequence of 16S ribosomal RNA gene has been deposited in GenBank (Acession number MT786342). Its producing siderophore was discovered based on chrome azurol S guide-screening of 12 isolates. One of them showed an orange halo appearance in the chrome azurol S agar plate. The fractionation with 50:50 (v/v) MeOH/water revealed the presence of serratiochelin A. The structure was then determined by MS 2 fragmentation.