Open AccessConstruction and Study of the Application of Novel Norfloxacin Fluorescent Probes
Author(s) -
Kuilin Lv,
Detian Wan,
Lei Wang,
Yuguang Lv
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/706/1/012034
Subject(s) - norfloxacin , fluorescence , excitation wavelength , chemistry , wavelength , analytical chemistry (journal) , chromatography , materials science , optics , optoelectronics , biochemistry , physics , ciprofloxacin , antibiotics
In this experiment, a fluorescent probe of tricyanohydrofuran-norfloxacin was successfully constructed. Fluorescence spectrophotometer was used to detect the fluorescence intensity of the system under the condition of the excitation wavelength was 362nm and the emission wavelength was 448nm. The optimal experimental conditions were optimized (the concentration of the complex, the amount of norfloxacin, pH, temperature and reaction time). The optimal concentration of the complex in the system is in the range of 1.0×10 −3 -3.5×10 −4 mol·L −1 , the dosage was 0.6mL, and optimal dosage of norfloxacin was 3.0 ml, the optimum pH of the system was 5.5, under this condition the fluorescence intensity of the system was the strongest. At the same time, the intramolecular charge transfer reaction mechanism of fluorescent probe was studied. And testing the content of norfloxacin capsules, the results were satisfactory. A new testing method of norfloxacin content successfully created.