Open AccessAnalysis of Three-dimensional Fluorescence Characteristics of Extracellular Polymeric Substances at Internal Points in MPR at Low Temperature
Author(s) -
Xi Tian,
Yanhong Lv,
Lei Ying,
Xuejiao Li,
Zhiming Ren,
Xin Liu,
Dejun Bian
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/706/1/012027
Subject(s) - extracellular polymeric substance , fluorescence , chemistry , excitation , fluorescence spectroscopy , analytical chemistry (journal) , extracellular , wavelength , bioreactor , matrix (chemical analysis) , materials science , chromatography , biofilm , biochemistry , optics , organic chemistry , optoelectronics , physics , biology , quantum mechanics , bacteria , genetics
Through three-dimension excitation emission matrix (EEM) fluorescence spectroscopy and fluorescence regional integration (FRI), the content and composition changes of extracellular polymeric substances (EPS) in different regions in the Micro-Pressure Inner-Loop Bioreactor (MPR) were analyzed, and the law of EPS in spatial points was found. The analysis shows that the high excitation wavelength tryptophan-like Peak T1 and the low excitation wavelength tyrosine-like Peak B appear in the spectra of tightly-bounded EPS (TB-EPS) and loosely-bounded EPS (LB-EPS) at different points. The content of protein substances in EPS is relatively high. In addition, the unique flow pattern in the reactor forms a multi-oxygen zone, which causes the total amount of EPS and the content of various organic substances, especially protein substances, to increase from the center of the reactor to the outside.