Open AccessIsolation and characterization genes in lobi-lobi (Flacourtia inermis) related to sugar metabolism
Author(s) -
Y W Silaban,
Ketty Suketi,
Slamet Susanto,
Deden Derajat Matra
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/694/1/012068
Subject(s) - invertase , biology , contig , sugar , sucrose , gene , genetics , biochemistry , genome
Lobi-lobi ( Flacourtia inermis ) is probably a native plant to the Moluccas (Maluku). In Indonesia, lobi-lobi fruit is used for traditional medicine because it has a phenolic compound. Sugar metabolism involved several genes that control the photosynthesis pathway. This research aims to isolate and characterize genes in which related to sugar metabolism in Lobi-lobi. The lobi-lobi contigs have 694 452 contigs produced by the velvet-assembled genome. The sugar-related genes were isolated from the assembled-genomes database against to SwissProt Database using the BLASTX program. The six-sugar related-genes were characterized involved 36 contigs of Sucrose-phosphate synthase (SPS), six contigs of Sucrose-phosphatase (SPP), 46 contigs of Sucrose synthase (SUS), 36 contigs of Alkaline/neutral invertase (INV), four contigs of Cytosolic invertase (CINV), and 17 contigs of Beta-fructofuranosidase (CWINV), respectively. Comprehensive research on sugar metabolism was used in this study. Then, the data also revealed the biological functions of these genes in flowering time control in Lobi-lobi.