Open AccessEffects of Commercial Antioxidants Applied in Ovo on Chorioallantoic Membrane and Putative Plasma Vitellogenin of Philippine Mallard (Anas platyrynchos L.)
Author(s) -
Renato S. A. Vega,
Christine B. Adiova,
John Sylvester Nas,
Desiree Joy V. Cerico,
D. D. Manalo
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/690/1/012026
Subject(s) - in ovo , vitellogenin , chorioallantoic membrane , vitamin e , positive control , body weight , endocrinology , biology , vitamin c , medicine , vitamin , chemistry , placebo , andrology , zoology , antioxidant , angiogenesis , embryo , biochemistry , traditional medicine , pathology , gene , alternative medicine , microbiology and biotechnology
Two experiments were conducted to evaluate the effects of different antioxidants and its overdose (4x) in ovo . Experiment 1 were grouped as vitamin C, MegaAntioxidant ® and Proflavanol C ® , while experiment 2 were grouped as 100, 200 and 400ppm of MegaAntioxidant ® having positive and negative control for both. The 200 fertile eggs in each experiment were injected with 100 ul/ egg of treatment solution and placebo (positive control), except negative control. Embryonic weight and body measurements were taken weekly (7-21 d). The anti-angiogenesis using CAM at day 4 was accomplished. Determination of putative vitellogenin (VTG) was done at 28 th day using SDS-PAGE. Both experiments showed comparable embryonic weight, body length, and head diameter indicating no deleterious effects of different antioxidants and MegaAntioxidants ® at high doses. Absence of red blemishes of liver was observed in three different antioxidants. The CAM resulted to lesser blood vessel formation significantly at 200 and 400 ppm MegaAntioxidant ® . Further, the 175 kDa putative VTG, was present in vitamin C, Proflavanol C ® , positive control and negative control, except MegaAntioxidant ® . The results imply that MegaAntioxidant ® having 32 different antioxidants could have protected the liver, spared VTG synthesis and had anti-angiogenic property at 200 and 400ppm doses.