Open AccessResults of primary screening of proteolytically active microorganisms that are promising for the transformation of organic pollution
Author(s) -
Н А Сидорова,
N. A. Chechkova
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/689/1/012041
Subject(s) - microorganism , bacilli , bacillus (shape) , biology , pseudomonas , bacteria , food science , microbiology and biotechnology , biomass (ecology) , antimicrobial , micrococcus , organic matter , chemistry , ecology , genetics
In order to optimize the methods of remediation of contaminated areas, strains of microorganisms were selected that are promising for the transformation of excess amounts and the accumulation of organic matter in surface water bodies. Research has been carried out on the isolation and selection of proteolytically active strains of microorganisms from the microflora of water. Among the isolated isolates, representatives of 3 genera dominated: Pseudomonas sp ., Micrococcus sp ., Bacillus sp., Which differed in the rate of biomass accumulation / optical density (OD), the number of viable cells (CFU/ml) and the concentration of residual protein (mg/ml). The highest proteolytic activity was found in representatives of the genus Bacillus sp . For bacilli, within 5 days of the experiment, a 7-fold increase in biomass was observed, and the number of viable cells increased 8-fold. Due to the proteolytic activity of Bacillus sp. the concentration of the protein in the test solution decreased from 0.2412 to 0.1123 mg/ml. The research carried out opens up prospects for the creation of biopreparations of targeted action based on mono- and polycultures of proteolytics for the purposes of ecobiotechnology.