Open AccessPreliminary study: Species identification of Rasbora sumatrana through the cytochrome oxidase subunit I DNA barcoding marker
Author(s) -
M Y Norfatimah,
Mohd Akram,
W. Z. A. Lyena,
Z. N. Aili,
A. Farizan
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/674/1/012107
Subject(s) - meristics , dna barcoding , biology , cytochrome c oxidase subunit i , phylogenetic tree , zoology , genetics , gene
Rasbora sumatrana or locally known as “seluang” in Malaysia is a pelagic fish. Unlike its other congeneric species is not categorized as an ornamental fish. Samples of the presumed R. sumatrana was obtained from an abandoned swamp in Hulu Langat, Selangor. Initial identification of the samples was conducted based on meristic and morphometric analyses, which showed congruence with R. sumatrana when referenced to FishBase. To complement the analysis, a molecular study was conducted based on the cytochrome oxidase subunit I (COI) DNA barcoding marker. Confirmation of the species identity was done by BLAST results with six voucher sequences of R. sumatrana and other Rasbora species from GenBank. The relationship of Rasbora spp. was analysed by phylogenetic and Automatic Barcode Gap Discovery (ABGD) analysis. Contradicting with morphological and meristic data analysis, both Maximum-Likelihood and Maximum Parsimony phylogenetic tree showed that the currently studied samples did not cluster with R. sumatrana . We believe that they may represent cryptic diversity and are tentatively classified as Rasbora spp.