Open AccessDNA barcode of Belodontichthys dinema from Indragiri and Tapung Rivers, Indonesia
Author(s) -
Roza Elvyra
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.179
H-Index - 26
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/674/1/012012
Subject(s) - genbank , dna barcoding , mega , phylogenetic tree , biology , cytochrome b , polymerase chain reaction , barcode , gene , genetic distance , mitochondrial dna , dna , cytochrome c oxidase subunit i , genetics , microbiology and biotechnology , zoology , genetic variation , physics , astronomy , computer science , operating system
Belodontichthys dinema with the local name of sengarat fish, is one of freshwater fish in Riau Province of Indonesia. B. dinema distributed in some rivers of Riau Province were Indragiri and Tapung rivers. This study reports analysis of DNA barcode of B. dinema using cytochrome c oxidase subunit I (COI) gene. The muscle of B. dinema fish using for DNA extraction. In this study had been obtained 652 bp of COI gene from Polymerase Chain Reaction (PCR) product. The Basic Local Alignment Search Tool (BLAST) analysis based on COI gene sequences shows that B. dinema from the Indragiri and Tapung rivers has 100% similarity with B. dinema from the existing database in GenBank. There are 14 marker nucleotides can characterize the species B. dinema molecularly. The value of genetic distance between B. dinema from Indragiri and Tapung rivers with B. dinema from GenBank data is 0.00. The phylogenetic tree showed that B. dinema from Indragiri and Tapung rivers, formed one group with B. dinema from Genbank data with bootstrap value is 100%. These results confirmed that sengarat fish identified was B. dinema based on the COI gene as DNA barcode.