Open AccessCharacterisation and cytotoxicity assay of curcumin nanostructured lipid carrier on HeLa cells
Author(s) -
Rabima,
A. Oktamauri
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/667/1/012055
Subject(s) - zeta potential , hela , dispersity , curcumin , sonication , particle size , bioavailability , cytotoxicity , chemistry , mtt assay , nanoparticle , aqueous solution , chromatography , materials science , in vitro , nanotechnology , biochemistry , organic chemistry , pharmacology , medicine
Low bioavailability is major problem in the use of curcumin. Nanostructured Lipid Carrier (NLC) is an interesting generation of lipid-based nanoparticles, because of its ability to increase the bioavailability of drugs. The objectives of this study were to make a formulation of curcumin in NLC (CRM-NLC) preparations, to determine its characteristic and to examine the cytotoxic effects of CRM-NLC on HeLa cells in vitro. CRM-NLC was made by the method of evaporation and diffusion of solvents in aqueous systems then followed by ultrasonication. Its particle size, polydispersity index, zeta potential were determined by using Particle Size Analyzer. The structure and morphology were observed by using Transmission Electron Microscopy then its cytotoxic activity toward HeLa cells was examined by using the MTT method then Half Maximal Inhibition Concentration (IC 50 ) was determined. CRM-NLC produced in this study had an average particle size of 17.4 nm, a polydispersity index of 0.574, the zeta potential of -63.43 mV, with structure and the morphology of CRM-NLC was round and smooth surface. CRM-NLC IC50 value obtained in this study was 8,872 μg / mL. This study has succeeded in making CRM-NLC preparation with good characteristics and improving curcumin activity on HeLa cells.