Open AccessHPLC-Based Quantitative Detection of Tylosin in Soil
Author(s) -
Kang Wang,
Shao-Peng Yu,
Jia Zhou
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/657/1/012035
Subject(s) - chromatography , high performance liquid chromatography , extraction (chemistry) , tylosin , elution , chemistry , acetonitrile , ammonium hydroxide , methanol , ammonium carbonate , aqueous solution , detection limit , ultrapure water , solid phase extraction , materials science , nuclear chemistry , biochemistry , organic chemistry , nanotechnology , antibiotics
The sample was extracted under the ultrasonic assistance and purified through the solid-phase extraction, and then the residual tylosin in soil was detected via the high-performance liquid chromatography (HPLC). Acetonitrile-aqueous solution was used as the extracting agent in the ultrasound-assisted extraction. The HLB solid-phase extraction column was activated using 5 mL of methanol and 5 mL of ultrapure water, leached with 5 mL of water and 5 mL of 2% ammonium hydroxide + 5% methanol solution, and eluted with 5 mL of methanol in the end. The determination was implemented using the HPLC method, where the detected wavelength λ value was 290 nm, the sample size was 20μL, and the column temperature was 30°C. Acetonitrile -0.01 mol/L monopotassium phosphate solution (pH=2.5) = 60:40 (V: V) was taken as the mobile phase in the detection of response degree to tylosin in soil. The detection results were as follows: LOD was 0.019mg/kg, LOQ was 0.062mg/kg, the coefficient of variation of intra-day precision was smaller than 10%, and that of inter-day precision was smaller than 15%.