Open AccessIntrogession of powdery mildew resistance into cultural pea from wild accession of P. fulvum
Author(s) -
С В Бобков,
Т. Н. Селихова
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/650/1/012091
Subject(s) - powdery mildew , biology , backcrossing , hybrid , introgression , population , botany , plant disease resistance , horticulture , genetics , gene , demography , sociology
Wildlife pea specimen P. fulvum i-609881 of the UIP collection (Saint Petersburg, Russia) in a greenhouse box has demonstrated full resistance to powdery mildew. As a result of crossing with cultivated peas, interspecific hybrids of Stabil × i-6098881 were obtained. In populations of interspecific hybrids, the BC 2 F 3 Stabil × i-609881 line of the leafless morphotype ( af ), resistant to the powdery mildew pathogen, was isolated as a result of backcrossing. As a result of crossing plants of this line with susceptible plants of the leafy ( Af ) variety Temp, the following series of pea hybrids was obtained. Analysis of F 1 hybrids showed that all plants were characterized by resistance to the pathogen powdery mildew. In the population of F 2 hybrids, a splitting analysis was performed on the basis of resistance to powdery mildew. The analysis revealed that the hybrid population consisted of 66 resistant and 20 affected plants. The actual splitting corresponded to the expected one for monogenic inheritance with a phenotype ratio of 3: 1 phenotype ratio (η 05 2 test, p=0.7087438). Based on the analysis of populations of F 1 and F 2 hybrids, it is concluded that resistance to powdery mildew is the result of introgression of the genetic resistance factor from the genome of the P. fulvum i-609881 sample and is encoded by the dominant allele of a single gene.