Open AccessPreliminary study of methane oxidizing bacteria isolation and selection on three rice agroecosystems
Author(s) -
Terry Ayu Adriany,
Amelia Santoso,
Alina Akhdiya,
A. Wihardjaka
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/648/1/012173
Subject(s) - agroecosystem , paddy field , methane , bacteria , agronomy , greenhouse gas , environmental science , oxidizing agent , agriculture , environmental chemistry , biology , chemistry , ecology , genetics , organic chemistry
The agricultural sector contributes to releasing methane (CH 4 ) as greenhouse gas emissions from lowland rice cultivation. One effort to reduce CH 4 emissions in paddy fields is the utilization of methane-oxidizing bacteria from paddy fields. The study aimed to obtain isolates of methane oxidizing bacteria from isolating and selecting their oxidation ability on three rice agroecosystems. The research was conducted at the Laboratory of the Indonesian Agricultural Environment Research Institute (IAERI), Pati, Central Java, Indonesia. Soil sampling was carried out in three rice agroecosystems, namely technical irrigation (SI), rainfed (TH), and organic rice cultivation (OF) rice fields in Pati Regency. Isolation and purification of bacteria used Nitrate Mineral Salts (NMS) with 1% methanol. Parameters collected were characteristics of morphology colonies, staining of gram bacteria and measuring methane oxidation ability. The results obtained 15 bacterial isolates from three different rice agroecosystems. The highest percentage of CH 4 concentration reduction from three rice different agroecosystems during 15 days of incubation were SI5 16, TH6 23, and OF3 23%, respectively. Methane oxidizing bacteria are expected as a technology to reduce CH 4 emissions from rice fields.