Open AccessAccount of the uncertainty of measurements of the degree of free radical oxidation in wheat germ by the method of chemiluminescent analysis
Author(s) -
O A Orlovtseva,
И. А. Никитин,
N L Kleimenova,
M V Klokonos,
V V Portnov,
S A Gavrilin
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/640/2/022065
Subject(s) - chemiluminescence , degree (music) , product (mathematics) , chemistry , mathematics , statistics , biological system , chromatography , physics , geometry , acoustics , biology
Uncertainty is a more objective concept for evaluating the accuracy of measuring product or process indicators than error. The article studies the adequacy of the assessment of the process of free radical oxidation in wheat germ by chemiluminescent analysis (CLA). This process in the product is due to the conjugated action of enzymes: lipase, lipoxygenase and catalase. To confirm the close relationship between their activity and the CLA parameters (the maximum value of the intensity and the slope of the kinetic curve), a linear correlation coefficient was calculated. As a result, a direct close relationship was established between the studied parameters, which confirms the possibility of assessing the degree of oxidation in the product by the CLA method. An algorithm for obtaining the uncertainty value is formed, which includes four main stages: a description of the measured values (the intensity of the peroxide processes, the inhibitory ability in the product); identification of sources of uncertainty (related to instrumental, methodological and subjective reasons, as well as to a random component); simplification of components to eliminate mutually compensating effects and calculation of uncertainties. The calculation of standard and expanded uncertainties was performed. Their assessment led to the conclusion about a high degree of accuracy of the obtained results with a reliability of 95%.