Open AccessEffect of Benzilaminopurine and Kinetin for shoot multiplication of Indigofera (Indigofera zollingeriana Miq.) by in vitro culture
Author(s) -
J I Royani,
S. Chotimah,
RahajengPuji Utami,
Widia Sri Fatma,
Susiyanti Susiyanti,
Aisyah Fatmawaty
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/637/1/012053
Subject(s) - kinetin , shoot , germination , biology , forage , legume , callus , botany , horticulture , in vitro , tissue culture , biochemistry
Indigofera ( Indigofera zollingeriana Miq.) is one of legume trees that useful as forage and have advantages in production and quality of forage compared to other types of legumes. This forage contains high protein and mineral sources, good fiber structure and high digestibility value. Nutrient contents are crude protein (31%) with 76% of DMD and 83% of OMD. Indigofera has low germination due to thick seed coat and fungal invasion during germination. Propagation by in vitro culture promise to multiply superior seeds from Indigofera. The aim for this research was to determine the effect of Benzylaminopurine (BAP) and Kinetin for multiply of shoots of Indigofera. The research was designed using Completely Randomized Design (CRD) with 2 factors (BAP and Kinetin concentration) and repeated 3 times. BAP concentration consists of four levels, 0, 1.0, 1.5 and 2 mg/L and Kinetin concentration consists of four levels 0, 1.0, 2 and 3 mg/L. Results showed that BAP gave the best results for number of shoots parameter at any concentration. Compact callus appears in basal of shoots at BAP (1.5 mg/L and 2 mg/L) media. Whereas media with Kinetin showed no significant effect on all parameters and there was no interaction between BAP and Kinetin.