Open AccessExperimental study of excessively-long translocation time of single DNA through sub-5 nanometer solid-state nanopores
Author(s) -
Xiaoqin Zhu,
Xiaojie Li,
Chaoming Gu,
Yang Liu
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/632/5/052072
Subject(s) - nanopore , dwell time , nanometre , materials science , nanopore sequencing , nanotechnology , chromosomal translocation , transmission electron microscopy , membrane , optoelectronics , biophysics , analytical chemistry (journal) , dna , chemistry , composite material , medicine , clinical psychology , biochemistry , biology , dna sequencing , gene , chromatography
Excessively-long translocation events of single DNAs are experimentally observed using a small nanopore. Solid-state Nano pores on SiNx membranes with pore diameters less than 5nm are fabricated via Transmission Electron Microscopy. The translocation testing system is set up based on patch-clamp and Lab-on-Chip, and translocation experiments of Lambda DNAs are conducted. Stable current traces and single molecular translocation events are achieved. Statistical analysis under various cross-membrane voltages shows typical characteristics of SiNx Nano pores, including event rates, threshold voltages, and noise power-spectrum-density. Particularly, excessively-long dwell time (>100ms) events through <5 nm nanopore are observed and attributed to the interaction between DNAs and pore walls. This characteristic is compared against the basic current-blockage model as well as that of a 10 nm nanopore control experiment, demonstrating additional blockage effects.