Open AccessDevelopment and Application of the Sample Support in Transmission Electron Microscopy
Author(s) -
Hengyu Pan
Publication year - 2021
Publication title -
iop conference series. earth and environmental science
Language(s) - English
Resource type - Journals
eISSN - 1755-1307
pISSN - 1755-1315
DOI - 10.1088/1755-1315/632/2/022004
Subject(s) - workflow , sample (material) , nanotechnology , process (computing) , computer science , cryo electron microscopy , resolution (logic) , materials science , physics , nuclear magnetic resonance , artificial intelligence , database , thermodynamics , operating system
Cryogenic electron microscopy is becoming an increasingly popular technology that allows us to observe the molecules in high-resolution details. With this refined way to study the structures in scientific researches, the observation of the molecules will be expected to make improvements. The conventional methods, such as the X-ray crystallography and the nuclear magnetic resonance spectroscopy, provide the basic observation of ground-state molecules and the measurement of various small molecules. But both of them contain flaws discovered in the continuing development process. Therefore, the application of cryo-EM becomes a broader area to explore. In this review, we will give an overall understanding of the cryogenic electron microscopy. We will discuss the intention behind the invention of the cryo-EM and introduce the main workflow of the experiments. In addition, we will discuss the methods to prepare the sample in a well-condition and compare them among different groups of experiments. More importantly, we will discuss the application of the graphene in cryo-EM and evaluate possible improvements in future development.